One of the degradation product was identified as 1-(3,5-dihydroxyphenyl)-6′-hydroxy-l’-undecen-l0′-one by TOF-MS/MS analysis. In addition, MQ01 showed a prominent degradation capacity of ZEN both in liquid medium (94.1%) and in practical samples such as corn (27.9%) and distiller's dried grains with solubles (35.3%). Protein homology analysis showed that the two antagonistic components were chitin-binding protein and subtilisin, respectively. The antifungal substances were extracted, separated by native-PAGE and identified through MALDI-TOF-MS. verticillioides, Colletotrichum gloeosporioides, Microdochium nivale, F. These results suggest that members of the Lysobacter group might provide a new source as plant-associated microbes that display biocontrol activity. enzymogenes is among the first description of members of this genus as biocontrol agents of plant diseases. amyloliquefaciens MQ01 showed broad-spectrum antifungal activity against eight phytopathogens, namely F. Inclusion of N4-7 and C3 as strains of L.
Through co-culture with Fusarium graminearum, the bacterial strain MQ01 was isolated from wheat rhizosphere soil for its antifungal activity and identified as Bacillus amyloliquefaciens according to its morphological, physiological and biochemical characteristics and phylogenetic trees based on sequences of the 16 S rRNA and gyrB genes. Understanding and manipulating plant attributes to enhance biological control. To implement effective biocontrol, the contribution of specific genes, mechanisms and traits to the biocontrol performance of BCAs need to be characterized and explored in greater detail. The present work aimed to isolate a bacterium capable of antagonizing various phytopathogenic fungi and degrading ZEN effectively. Many physiological and microbial characteristics influence the biocontrol performance of the biological control agents (BCAs) in agricultural fields. Potential of Trichoderma species as biocontrol agent against Curvularia lunata causing fruit rot of tomato (Lycopersicum esculentum Mill. the ‘New Association’ or ‘Neoclassical’ approach targets native pests with non-native biological control. ‘Classical’ biocontrol targets a non-native pest with one or more species of biocontrol agents from the pest’s native range 2. Fusarium graminearum is the predominant pathogen related to Fusarium head blight (FHB) which causes mycotoxin contamination of grains, mainly involving zearalenone (ZEN) and deoxynivalenol. Plant characters that appeared to make plants more susceptible to biological control included genetic uniformity, lack of perennating organs, and susceptibility. There are several general approaches to using biocontrol agents: 1.